علوم زیستی دریا
Mehrnoush Norouzi
Abstract
Genetic structure of golden mullet, Liza aurata, investigated in the Gomishan and Anzali wetlands using six microsatellite primer sets (Muce55, Muce37, Muso10, Muco16, Muso19, and Muso22). Totally 60 samples of adult golden mullet were collected from these regions. All primer sets as polymorphic loci ...
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Genetic structure of golden mullet, Liza aurata, investigated in the Gomishan and Anzali wetlands using six microsatellite primer sets (Muce55, Muce37, Muso10, Muco16, Muso19, and Muso22). Totally 60 samples of adult golden mullet were collected from these regions. All primer sets as polymorphic loci were used to analyze the genetic variation. Analyses revealed that average of alleles (Na) per locus was 5.3 (range 3 to 9 alleles) and in regions, samples of Gomishan wetland 5.1 and Anzali wetland 5.5 respectively. All sampled regions contained private alleles. The average estimates of inbreeding coefficients (Fis and Fit) values of 6 microsatellites were positive. The average observed and expected heterozygosity was in Gomishan wetland 0.153 and 0.721 and Anzali wetland 0.328 and 0.747 respectively. Deviations from Hardy-Weinberg equilibrium were in all cases (P
Mehrnoush Norouzi; Ali Nazemi; Fatemeh Daneshvar; Mohammad Hadi Samiei
Volume 12, Issue 1 , April 2013, , Pages 11-20
Abstract
Common kilka, Clupeonella cultriventris is an economic fish in Caspian sea and we investigate population genetic structure of common kilka in the South Caspian Sea costline (Mazandaran province) using microsatellite markers. Totally, 60 individuals of adult common kilka from two seasons were sampled. ...
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Common kilka, Clupeonella cultriventris is an economic fish in Caspian sea and we investigate population genetic structure of common kilka in the South Caspian Sea costline (Mazandaran province) using microsatellite markers. Totally, 60 individuals of adult common kilka from two seasons were sampled. Eight sets of microsatellite primers were developed from American shad and Pacific herring tested on genomic DNA of common kilka. At this point only the five successfully used primer sets and were used to analyze the genetic variation in adultsof the common kilka population. Analyses revealed that average of alleles per locus was 11.7 (Na range 6 to 17 alleles per locus). Both of sampled seasons contained private alleles. Average observed and expected heterozygosity were 0.543 and 0.866 respectively. Deviations from Hardy-Weinberg equilibrium were in most cases (P<0.01). Basis on AMOVA for both FST values among pairs them indicated a significant difference between the two seasons (P≤0.01). These results support the existence of different genetic populations along the South Caspian Sea costline (Mazandaran province) in different seasons.